Anti-EVC2 antibody (ab198930)
Key features and details
- Rabbit polyclonal to EVC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EVC2 antibody
See all EVC2 primary antibodies -
Description
Rabbit polyclonal to EVC2 -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total EVC2 protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human EVC2 (internal sequence). Gene Accession: NP_667338
Database link: Q86UK5 -
Positive control
- Lysate from human placenta tissue; Human colon cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab198930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200 - 1/1000. Predicted molecular weight: 148 kDa. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
EVC2 is an integral membrane protein that plays a vital role in bone formation and skeletal development. Defects in EVC2 are a cause of Ellis-van Creveld syndrome (EVC), also known as chondroectodermal dysplasia. EVC is an autosomal recessive disorder characterized by the clinical tetrad of chondrodystrophy, polydactyly, ectodermal dysplasia and cardiac anomalies. Patients manifest short-limb dwarfism, short ribs, postaxial polydactyly and dysplastic nails and teeth. Congenital heart defects, most commonly an atrioventricular septal defect, are observed in 60% of affected individuals. Defects in Defects in EVC2 are also a cause of acrofacial dysostosis Weyers type (WAD), also known as Curry-Hall syndrome. Acrofacial dysostoses are a heterogeneous group of disorders combining limb defects with facial abnormalities. WAD is an autosomal dominant disorder characterized by dysplastic nails, postaxial polydactyly, acrofacial dysostosis, short limbs and short stature. The phenotype is milder than Ellis-van Creveld syndrome. -
Cellular localization
Membrane; Multi-pass membrane protein -
Database links
- Entrez Gene: 132884 Human
- Entrez Gene: 68525 Mouse
- Omim: 607261 Human
- SwissProt: Q86UK5 Human
- SwissProt: Q8K1G2 Mouse
- Unigene: 87306 Human
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Alternative names
- Ellis van Creveld syndrome 2 antibody
- LBN antibody
- Limbin antibody
Images
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Western blot - Anti-EVC2 antibody (ab198930)Anti-EVC2 antibody (ab198930) at 1/450 dilution + human placenta tissue lysate at 40 µg
Predicted band size: 148 kDa
Exposure time: 7 minutesGel: 6% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVC2 antibody (ab198930)Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling EVC2 with ab198930 at a 1/40 dilution.
Protocols
Datasheets and documents
References (0)
ab198930 has not yet been referenced specifically in any publications.
