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Date: October 4-6, 2021
SBI2 is an international community of leaders, scientists, and students promoting technological advancement, discovery, and education to quantitatively interrogate biological models to provide high context information at the cellular level. Join Abcam this year at the 8th annual conference of SBI2 which will take virtually October 4-6th.
Abcam’s expertise supports the creation of high-quality products that meet our global customers’ complex research requirements, including high-throughput immunoassays and custom antibody services. Our world-leading immunoassays and antibodies are essential across multiple applications within drug discovery, diagnostics, and basic research. We partner with our customers, listening closely to their needs, to produce unique, tailor-made solutions.
Accelerating high-throughput screening using FirePlex®-384 multiplexing particle technology with High Content Imaging and laboratory automation
FirePlex-384 is a new multiplex assay platform which combines the rapid data harvesting power of multiplexing, with standard High-Content Imaging instrumentation in an easy to use automatable high-throughput platform High-throughput screening (HTS) technologies play an essential role in drug discovery where rapid querying of large numbers of biological samples for biomarkers or compound effects guides development decisions. Multiplexing is a highly effective tool for significantly increasing screening throughput and efficiency, but technically challenging and expensive to implement into HTS workflows. Closed multiplexing platforms with dedicated instrumentation and complicated workflows also present obstacles for adoption.
FirePlex-384 multiplex platform addresses these obstacles using patented FirePlex hydrogel particle technology. The three-region encoded particle design provides sensitive and reproducible 10 analyte in-well multiplexing for a range of assay applications. Assay data acquisition is conducted on standard High-Content Imagers, and data analysis is achieved with the FirePlex Analysis Workbench software, eliminating the need for purchase of an expensive dedicated instrument and software licenses. Easy to use optional two-step workflow or no-wash assay workflows limit hands-on time and are compatible with high-throughput liquid handling robotic laboratory automation. FirePlex-384 catalogue immunoassays offer up to 10 multiplexed protein analytes on pre-designed content panels or flexible Mix & Match pools for custom configurations.
The merging of high-throughput systems with multiplexing assay technologies provides a method for biomarker efficiently screening very large numbers of biological samples. Combining these two technologies is complicated and expensive. FirePlex-384 addresses the need for a sensitive and reliable multiplex assay platform that is compatible with high-throughput workflows and existing instrumentation, accelerating biomarker or compound screening for drug discovery easily and inexpensively.