Key features and details
- Rabbit polyclonal to EVER2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-EVER2 antibody
DescriptionRabbit polyclonal to EVER2
SpecificityAt least three isoforms of EVER2 are known to exist; this antibody will only recognize the two larger isoforms. The antibody has no cross reactivity to EVER1.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid synthetic peptide from near the center of human EVER2.
- Jurkat cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).|
RelevanceEpidermodysplasia verruciformis (EV) is an autosomal recessive genodermatosis associated with a high risk of skin cancers resulting from a high susceptibility to infection by specific human papillomaviruses. Mutations in two homologous genes EVER1 and EVER2 cause the majority of EV cases. These two proteins form a complex and interact with the zinc transporter ZnT-1 in the endoplasmic reticulum. Cells lacking EVER2 accumulated higher levels of zinc in the nucleolus and nucleus compare to those cells with and intact EVER2 gene, indicating that one role of EVER2 is to regulate the intracellular distribution of zinc. At least two isoforms of EVER2 are known to exist.
Cellular localizationEndoplasmic reticulum membrane; Multi pass membrane protein.
- epidermodysplasia verruciformis 2 antibody
- Epidermodysplasia verruciformis protein 2 antibody
- EV2 antibody
Lane 1 : Anti-EVER2 antibody (ab69859) at 1 µg/ml
Lane 2 : Anti-EVER2 antibody (ab69859) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 82 kDa
Observed band size: 70,82 kDa why is the actual band size different from the predicted?
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
ab69859 at 5 µg/ml staining EVER2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Immunofluorescence of EVER2 in Human Brain cells using ab69859 at 20 ug/ml.
ab69859 has not yet been referenced specifically in any publications.