Recombinant
RabMAb

Anti-EVI1 antibody [EPR5996] (ab124934)

Overview

  • Product name
    Anti-EVI1 antibody [EPR5996]
    See all EVI1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5996] to EVI1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human EVI1 aa 300-400. The exact sequence is proprietary.

  • Positive control
    • HeLa and SKOV 3 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Relevance
      EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • AML1 EVI 1 antibody
      • AML1 EVI 1 fusion protein antibody
      • AML1 EVI1 antibody
      • Ecotropic viral integration site 1 antibody
      • Ecotropic virus integration 1 site protein antibody
      • Ecotropic virus integration site 1 protein homolog antibody
      • EVI 1 antibody
      • EVI1 antibody
      • MDS1 and EVI1 complex locus antibody
      • MDS1 and EVI1 complex locus protein EVI1 antibody
      • MDS1 and EVI1 complex locus protein MDS1 antibody
      • MDS1 antibody
      • MDS1 EVI1 antibody
      • MGC163392 antibody
      • MGC97004 antibody
      • Myelodysplasia syndrome 1 antibody
      • Myelodysplasia syndrome 1 protein antibody
      • Myelodysplasia syndrome-associated protein 1 antibody
      • Oncogene EVI1 antibody
      • PRDM3 antibody
      • Zinc finger protein Evi1 antibody
      see all

    Images

    • All lanes : Anti-EVI1 antibody [EPR5996] (ab124934) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKOV 3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution

      Predicted band size: 118 kDa
      Observed band size: 145,200 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 105 kDa (possible cleavage fragment)

    References

    ab124934 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124934.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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