Recombinant Anti-EVI1 antibody [EPR5996] (ab124934)
![Western blot - Anti-EVI1 antibody [EPR5996] (ab124934)](https://www.abcam.com/ps/products/124/ab124934/Images/ab124934-138964-anti-evi1-antibody-epr5996-western-blot.jpg "Western blot - Anti-EVI1 antibody [EPR5996] (ab124934)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5996] to EVI1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-EVI1 antibody [EPR5996]
See all EVI1 primary antibodies -
Description
Rabbit monoclonal [EPR5996] to EVI1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human EVI1 aa 300-400. The exact sequence is proprietary.
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Positive control
- HeLa and SKOV 3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5996 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa). |
Target
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Relevance
EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2122 Human
- Omim: 165215 Human
- SwissProt: Q03112 Human
- SwissProt: Q13465 Human
- Unigene: 744090 Human
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Alternative names
- AML1 EVI 1 antibody
- AML1 EVI 1 fusion protein antibody
- AML1 EVI1 antibody
see all
Images
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Western blot - Anti-EVI1 antibody [EPR5996] (ab124934)All lanes : Anti-EVI1 antibody [EPR5996] (ab124934) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKOV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 118 kDa
Observed band size: 145,200 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible cleavage fragment) -
![Anti-EVI1 antibody [EPR5996] (ab124934)](https://www.abcam.com/ps/products/124/ab124934/Images/ab124934-2-benefits-of-recombinant-antibodies.png)
Anti-EVI1 antibody [EPR5996] (ab124934)
Datasheets and documents
References (3)
ab124934 has been referenced in 3 publications.
- Luciani GM et al. Characterization of inv(3) cell line OCI-AML-20 with stroma-dependent CD34 expression. Exp Hematol 69:27-36 (2019). PubMed: 30352278
- Ma H et al. PBK, targeted by EVI1, promotes metastasis and confers cisplatin resistance through inducing autophagy in high-grade serous ovarian carcinoma. Cell Death Dis 10:166 (2019). PubMed: 30778048
- Ivanochko D et al. Direct interaction between the PRDM3 and PRDM16 tumor suppressors and the NuRD chromatin remodeling complex. Nucleic Acids Res N/A:N/A (2018). PubMed: 30462309
