Recombinant Anti-EVI1 antibody [EPR5996] (ab124934)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-EVI1 antibody [EPR5996]
See all EVI1 primary antibodies -
Description
Rabbit monoclonal [EPR5996] to EVI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human EVI1 aa 300-400. The exact sequence is proprietary.
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Positive control
- HeLa and SKOV 3 cell lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5996 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa). | |
| ICC/IF | 1/50 - 1/100. |
Target
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Relevance
EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2122 Human
- Omim: 165215 Human
- SwissProt: Q03112 Human
- SwissProt: Q13465 Human
- Unigene: 744090 Human
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Alternative names
- AML1 EVI 1 antibody
- AML1 EVI 1 fusion protein antibody
- AML1 EVI1 antibody
see all
Images
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![Western blot - Anti-EVI1 antibody [EPR5996] (ab124934)](https://www.abcam.com/ps/products/124/ab124934/Images/ab124934-138964-anti-evi1-antibody-epr5996-western-blot.jpg)
Western blot - Anti-EVI1 antibody [EPR5996] (ab124934)All lanes : Anti-EVI1 antibody [EPR5996] (ab124934) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKOV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 118 kDa
Observed band size: 145,200 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible cleavage fragment)
Datasheets and documents
References
This product has been referenced in:
- Luciani GM et al. Characterization of inv(3) cell line OCI-AML-20 with stroma-dependent CD34 expression. Exp Hematol 69:27-36 (2019). Read more (PubMed: 30352278) »
- Ma H et al. PBK, targeted by EVI1, promotes metastasis and confers cisplatin resistance through inducing autophagy in high-grade serous ovarian carcinoma. Cell Death Dis 10:166 (2019). Read more (PubMed: 30778048) »
