Anti-EVI5L antibody (ab243722)
Key features and details
- Rabbit polyclonal to EVI5L
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EVI5L antibody -
Description
Rabbit polyclonal to EVI5L -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EVI5L aa 400-500.
Database link: Q96CN4 -
Positive control
- IHC-P: Human stomach tissue. WB: EVI5L overexpressed HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 91 kDa.
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Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 91 kDa. |
Target
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Relevance
EVI5L functions as a GTPase-activating protein (GAP) with a broad specificity. May interact with RAB10. -
Cellular localization
Intracellular -
Database links
- Entrez Gene: 115704 Human
- GenBank: NM_145245 Human
- SwissProt: Q96CN4 Human
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Alternative names
- Ecotropic viral integration site 5 like protein antibody
- ecotropic viral integration site 5-like antibody
- EVI5 like protein antibody
Images
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Formalin-fixed, paraffin-embedded human stomach tissue stained for EVI5L using ab243722 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-EVI5L antibody (ab243722) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : EVI5L overexpression HEK-293T whole cell lysate
Predicted band size: 91 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab243722 has been referenced in 1 publication.
- Zhang M et al. CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells. Genes (Basel) 11:N/A (2020). PubMed: 32605180