Key features and details
- Rabbit polyclonal to EVL
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EVL antibody
DescriptionRabbit polyclonal to EVL
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human tonsil tissue; U-2 OS cells.
This product was previously labelled as Enah/Vasp-like
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa.|
RelevanceEnah/Vasp-like (EVL) is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes that require dynamic actin remodeling such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. There are two different isoforms, generated by alternative splicing.
Cellular localizationCytoplasm, cytoskeleton. Lamellipodium.
- AI528774 antibody
- Ena-vasodilator stimulated phosphoprotein antibody
- Ena/vasodilator stimulated phosphoprotein like antibody
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling EVL with ab204835 at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling EVL with ab204835 at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling EVL with ab204835 at 1/1000 dilution.
Negative control: Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling EVL with ab204835 at 1/1000 dilution.
Negative control: Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EVL with ab204835 at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EVL with ab204835 at 1/1000 dilution.
Immunofluorescent analysis of PFA- fixed, Triton X-100 permeabilized U-2 OS cells labeling EVL with ab204835 at 4 µg/ml (green).
Anti-EVL antibody (ab204835) + MOLT-4
Predicted band size: 40 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
ab204835 has not yet been referenced specifically in any publications.