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Tags & Cell Markers Cell Type Markers Epi / Endo-thelial
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RecombinantRabMAb

Recombinant Anti-EVPL antibody [EPR14688] (ab185217)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
  • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)
  • Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)
  • Anti-EVPL antibody [EPR14688] (ab185217)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14688] to EVPL
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-EVPL antibody [EPR14688]
    See all EVPL primary antibodies
  • Description

    Rabbit monoclonal [EPR14688] to EVPL
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal skin and A431 cell lysates. Human skin tissue. HeLa cells.
  • General notes

     

     

    Previously labelled as Envoplakin. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14688
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Desmosomes

Associated products

  • Alternative Versions

    • Anti-EVPL antibody [EPR14688] - BSA and Azide free (ab250816)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).
Flow Cyt
Use at an assay dependent concentration.
Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).
Flow Cyt
Use at an assay dependent concentration.

Target

  • Relevance

    EVPL is a component of the cornified envelope (epidermal barrier) and a member of the plakin family of cytoskeletal linker proteins. It is exclusively expressed in stratified squamous epithelia and is induced during differentiation of epidermal keratinocytes.
  • Cellular localization

    Cell junction; desmosome. Note=Colocalized with DSP at desmosomes and along intermediate filaments.
  • Database links

    • Entrez Gene: 2125 Human
    • Omim: 601590 Human
    • SwissProt: Q92817 Human
    • Unigene: 500635 Human
    • Alternative names

      • 210 kDa cornified envelope antibody
      • 210 kDa cornified envelope precursor protein antibody
      • 210 kDa paraneoplastic pemphigus antigen antibody
      • Envoplakin antibody
      • EVPK antibody
      • P210 antibody
      see all

    Images

    • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Anti-EVPL antibody [EPR14688] (ab185217) at 1/1000 dilution + Human fetal skin lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 232 kDa

    • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Anti-EVPL antibody [EPR14688] (ab185217) at 1/10000 dilution + A431 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 232 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling EVPL with ab185217 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)
      Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)

      Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling EVPL with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    • Anti-EVPL antibody [EPR14688] (ab185217)
      Anti-EVPL antibody [EPR14688] (ab185217)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab185217? Please let us know so that we can cite the reference in this datasheet.

    ab185217 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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