RecombinantRabMAb

Recombinant Anti-EVPL antibody [EPR14688] (ab185217)

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Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
  • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)
  • Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)
  • Anti-EVPL antibody [EPR14688] (ab185217)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14688] to EVPL
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-EVPL antibody [EPR14688]
    See all EVPL primary antibodies

  • Description

    Rabbit monoclonal [EPR14688] to EVPL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal skin and A431 cell lysates. Human skin tissue. HeLa cells.

  • General notes

     

     

    Previously labelled as Envoplakin. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).
Flow Cyt
Use at an assay dependent concentration.
Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).
Flow Cyt
Use at an assay dependent concentration.

Target

  • Relevance

    EVPL is a component of the cornified envelope (epidermal barrier) and a member of the plakin family of cytoskeletal linker proteins. It is exclusively expressed in stratified squamous epithelia and is induced during differentiation of epidermal keratinocytes.

  • Cellular localization

    Cell junction; desmosome. Note=Colocalized with DSP at desmosomes and along intermediate filaments.

  • Database links

    • Alternative names

      • 210 kDa cornified envelope antibody
      • 210 kDa cornified envelope precursor protein antibody
      • 210 kDa paraneoplastic pemphigus antigen antibody
      • Envoplakin antibody
      • EVPK antibody
      • P210 antibody
      see all

    Images

    • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Anti-EVPL antibody [EPR14688] (ab185217) at 1/1000 dilution + Human fetal skin lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 232 kDa

    • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
      Anti-EVPL antibody [EPR14688] (ab185217) at 1/10000 dilution + A431 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 232 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling EVPL with ab185217 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)
      Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)

      Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling EVPL with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    • Anti-EVPL antibody [EPR14688] (ab185217)
      Anti-EVPL antibody [EPR14688] (ab185217)

    References (0)

    Publishing research using ab185217? Please let us know so that we can cite the reference in this datasheet.

    ab185217 has not yet been referenced specifically in any publications.

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