Overview

  • Product name

    Anti-EVPL antibody [EPR14688]
    See all EVPL primary antibodies
  • Description

    Rabbit monoclonal [EPR14688] to EVPL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human EVPL aa 1500-1650. The exact sequence is proprietary.
    Database link: Q92817

  • Positive control

    • Human fetal skin and A431 cell lysates. Human skin tissue. HeLa cells.
  • General notes

     

     

    Previously labelled as Envoplakin. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    EVPL is a component of the cornified envelope (epidermal barrier) and a member of the plakin family of cytoskeletal linker proteins. It is exclusively expressed in stratified squamous epithelia and is induced during differentiation of epidermal keratinocytes.
  • Cellular localization

    Cell junction; desmosome. Note=Colocalized with DSP at desmosomes and along intermediate filaments.
  • Database links

  • Alternative names

    • 210 kDa cornified envelope antibody
    • 210 kDa cornified envelope precursor protein antibody
    • 210 kDa paraneoplastic pemphigus antigen antibody
    • Envoplakin antibody
    • EVPK antibody
    • P210 antibody
    see all

Images

  • Anti-EVPL antibody [EPR14688] (ab185217) at 1/1000 dilution + Human fetal skin lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 232 kDa

  • Anti-EVPL antibody [EPR14688] (ab185217) at 1/10000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 232 kDa

  • Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling EVPL with ab185217 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling EVPL with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab185217 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185217.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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