Recombinant Anti-EVPL antibody [EPR14688] (ab185217)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14688] to EVPL
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-EVPL antibody [EPR14688]
See all EVPL primary antibodies -
Description
Rabbit monoclonal [EPR14688] to EVPL -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal skin and A431 cell lysates. Human skin tissue. HeLa cells.
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General notes
Previously labelled as Envoplakin.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14688 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IHC-P |
Human
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WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).
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|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa). |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Relevance
EVPL is a component of the cornified envelope (epidermal barrier) and a member of the plakin family of cytoskeletal linker proteins. It is exclusively expressed in stratified squamous epithelia and is induced during differentiation of epidermal keratinocytes. -
Cellular localization
Cell junction; desmosome. Note=Colocalized with DSP at desmosomes and along intermediate filaments. -
Database links
- Entrez Gene: 2125 Human
- Omim: 601590 Human
- SwissProt: Q92817 Human
- Unigene: 500635 Human
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Alternative names
- 210 kDa cornified envelope antibody
- 210 kDa cornified envelope precursor protein antibody
- 210 kDa paraneoplastic pemphigus antigen antibody
see all
Images
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Anti-EVPL antibody [EPR14688] (ab185217) at 1/1000 dilution + Human fetal skin lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 232 kDa -
Anti-EVPL antibody [EPR14688] (ab185217) at 1/10000 dilution + A431 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 232 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling EVPL with ab185217 at 1/1000 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling EVPL with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Protocols
References (0)
ab185217 has not yet been referenced specifically in any publications.