Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14688] to EVPL - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
Product nameAnti-EVPL antibody [EPR14688] - BSA and Azide free
See all EVPL primary antibodies
DescriptionRabbit monoclonal [EPR14688] to EVPL - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human EVPL aa 1500-1650. The exact sequence is proprietary.
Database link: Q92817
Ab250816 is the carrier-free version of ab185217. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250816 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab250816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 210 kDa (predicted molecular weight: 232 kDa).|
RelevanceEVPL is a component of the cornified envelope (epidermal barrier) and a member of the plakin family of cytoskeletal linker proteins. It is exclusively expressed in stratified squamous epithelia and is induced during differentiation of epidermal keratinocytes.
Cellular localizationCell junction; desmosome. Note=Colocalized with DSP at desmosomes and along intermediate filaments.
- 210 kDa cornified envelope antibody
- 210 kDa cornified envelope precursor protein antibody
- 210 kDa paraneoplastic pemphigus antigen antibody
Anti-EVPL antibody [EPR14688] (ab185217) at 1/1000 dilution + Human fetal skin tissue lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 232 kDa
This data was developed using ab185217, the same antibody clone in a different buffer formulation.
This data was developed using ab185217, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling EVPL with ab185217 at 1/1000 dilution. The slide is counterstained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using ab185217, the same antibody clone in a different buffer formulation.Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling EVPL with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab250816 has not yet been referenced specifically in any publications.