Key features and details
- Rabbit polyclonal to EWI-2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EWI-2 antibody
DescriptionRabbit polyclonal to EWI-2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Synthetic peptide corresponding to Human EWI-2 aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Brain Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).|
FunctionMay play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
Tissue specificityExpressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.
Sequence similaritiesContains 4 Ig-like C2-type (immunoglobulin-like) domains.
DomainThe Ig-like C2-type domains 3 and 4 are required for interaction with CD81.
The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.
Cellular localizationCell membrane.
- Information by UniProt
- CD316 antibody
- CD316 antigen antibody
- CD81 partner 3 antibody
Anti-EWI-2 antibody (ab74890) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
EWI-2 contains a number of potential glycosylation sites and a phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab74890 has been referenced in 1 publication.
- Liang Y et al. Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J Biol Chem 289:32526-37 (2014). PubMed: 25261472