Overview

  • Product name

    Anti-EWI-2 antibody
  • Description

    Rabbit polyclonal to EWI-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human EWI-2 aa 450-550 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab74889)

  • Positive control

    • This antibody gave a positive signal in Human Brain Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab74890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
  • Tissue specificity

    Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.
  • Sequence similarities

    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.
    The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD316 antibody
    • CD316 antigen antibody
    • CD81 partner 3 antibody
    • CD81P3 antibody
    • EWI-2 antibody
    • EWI2 antibody
    • Glu-Trp-Ile EWI motif-containing protein 2 antibody
    • igsf-8 antibody
    • IgSF8 antibody
    • IGSF8_HUMAN antibody
    • Immunoglobulin superfamily member 8 antibody
    • Immunoglobulin superfamily member 8 precursor antibody
    • KCT-4 antibody
    • KCT4 antibody
    • Keratinocytes-associated transmembrane protein 4 antibody
    • LIR-D1 antibody
    • PGRL antibody
    • Prostaglandin regulatory-like protein antibody
    see all

Images

  • Anti-EWI-2 antibody (ab74890) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    EWI-2 contains a number of potential glycosylation sites and a phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:

  • Liang Y  et al. Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J Biol Chem 289:32526-37 (2014). Read more (PubMed: 25261472) »
See 1 Publication for this product

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