Anti-Exfoliative Toxin A/ETA antibody (ab225679)
Key features and details
- Rabbit polyclonal to Exfoliative Toxin A/ETA
- Suitable for: WB
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Overview
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Product name
Anti-Exfoliative Toxin A/ETA antibody -
Description
Rabbit polyclonal to Exfoliative Toxin A/ETA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Recombinant full length protein corresponding to Staphylococcus aureus Exfoliative Toxin A/ETA aa 1 to the C-terminus.
Database link: P09331 -
Positive control
- WB: Recombinant Staphylococcus aureus Exfoliative toxin A/ETA protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa). |
Target
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Relevance
Staphylococcus aureus Exfoliative Toxin A (ETA) has serine protease-like properties and binds to the skin protein profilaggrin. It cleaves substrates after acidic residues. Exfoliative toxins cause impetigous diseases commonly referred as staphylococcal scalded skin syndrome (SSSS). -
Database links
- Entrez Gene: 5330666 Staphylococcus aureus
- SwissProt: P09331 Staphylococcus aureus
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Alternative names
- Epidermolytic toxin A antibody
- Eta antibody
- Exfoliative toxin A antibody
Images
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All lanes : Anti-Exfoliative Toxin A/ETA antibody (ab225679) at 1/500 dilution
Lane 1 : Recombinant Staphylococcus aureus Exfoliative toxin A/ETA protein at 0.2 µg
Lane 2 : Recombinant Staphylococcus aureus Exfoliative toxin A/ETA protein at 0.1 µg
Lane 3 : Recombinant Staphylococcus aureus Exfoliative toxin A/ETA protein at 0.05 µg
Lane 4 : Recombinant Staphylococcus aureus Exfoliative toxin A/ETA protein at 0.025 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab225679 has not yet been referenced specifically in any publications.