Recombinant Anti-EXOC2 antibody [EPR9420] - BSA and Azide free (ab248907)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9420] to EXOC2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EXOC2 antibody [EPR9420] - BSA and Azide free
See all EXOC2 primary antibodies -
Description
Rabbit monoclonal [EPR9420] to EXOC2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, SH-SY5Y , Daudi, BxPC-3 cell lysates; Human placenta and Fetal brain lysates. Flow Cyt (intra): BxPC-3 cells.
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General notes
ab248907 is the carrier-free version of ab140620.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9420 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 104 kDa.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 104 kDa. |
Target
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Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. -
Tissue specificity
Widely expressed with highest levels in brain and placenta. -
Sequence similarities
Belongs to the SEC5 family.
Contains 1 IPT/TIG domain. -
Domain
Interacts with RALA through the TIG domain. - Information by UniProt
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Database links
- Entrez Gene: 55770 Human
- Entrez Gene: 66482 Mouse
- Entrez Gene: 171455 Rat
- Omim: 615329 Human
- SwissProt: Q96KP1 Human
- SwissProt: Q9D4H1 Mouse
- SwissProt: O54921 Rat
- Unigene: 484412 Human
see all -
Alternative names
- EXOC2 antibody
- EXOC2_HUMAN antibody
- Exocyst complex component 2 antibody
see all
Images
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All lanes : Anti-EXOC2 antibody [EPR9420] (ab140620) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EXOC2 CRISPR/Cas9 edited HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 104 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab140620).
Lanes 1- 4: Merged signal (red and green). Green - ab140620 observed at 104 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab140620 was shown to react with EXOC2 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab265839 (CRISPR/Cas9 edited cell lysate ab257944) lane below 104kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EXOC2 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab140620 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-EXOC2 antibody [EPR9420] (ab140620) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : Fetal brain tissue lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 104 kDaThis data was developed using ab140620, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248907 has not yet been referenced specifically in any publications.