Western blot abreview for Anti-Exonuclease 1 antibody

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Abreviews
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (CV-1)
Loading amount
30 µg
Specification
CV-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/500
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS-Tween

Secondary antibody

Name
Non-Abcam antibody was used: Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 130 kDa Non-specific: 70, 65 kDa

Additional data

Abcam response
Thank you for your review of this previously untested species. We do not have an explanation for the 70 kDa band, assuming it is not a fragment resulting from proteolytic degradation.

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Verified customer

Submitted Oct 16 2012

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