Anti-Exonuclease 1 antibody (ab155553)
Key features and details
- Rabbit polyclonal to Exonuclease 1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Exonuclease 1 antibody
See all Exonuclease 1 primary antibodies -
Description
Rabbit polyclonal to Exonuclease 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 474-753 of Human Exonuclease 1 (Uniprot ID: Q9UQ84).
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Positive control
- Jurkat and Mouse thymus whole cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 94 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 94 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. -
Tissue specificity
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. -
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. -
Developmental stage
Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus. -
Post-translational
modificationsPhosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein. -
Cellular localization
Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase. - Information by UniProt
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Database links
- Entrez Gene: 9156 Human
- Entrez Gene: 26909 Mouse
- Omim: 606063 Human
- SwissProt: Q9UQ84 Human
- SwissProt: Q9QZ11 Mouse
- Unigene: 498248 Human
- Unigene: 283046 Mouse
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Alternative names
- exo1 antibody
- EXO1_HUMAN antibody
- ExoI antibody
see all
Images
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Anti-Exonuclease 1 antibody (ab155553) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 94 kDa
7.5% SDS PAGE -
Anti-Exonuclease 1 antibody (ab155553) at 1/1000 dilution + Mouse thymus whole cell lysate at 50 µg
Predicted band size: 94 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling Exonuclease 1 with ab155553 at 1/500 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155553 has not yet been referenced specifically in any publications.