Product nameAnti-Exonuclease 1 antibody
See all Exonuclease 1 primary antibodies
DescriptionRabbit polyclonal to Exonuclease 1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HeLa and 293T whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95068 was affinity purified using an epitope specific to Exonuclease 1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab95068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 94 kDa.|
|IP||Use at 5-15 µg/mg of lysate.|
Function5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
Tissue specificityHighly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
Developmental stageHighly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
modificationsPhosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
Cellular localizationNucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
- Information by UniProt
- exo1 antibody
- EXO1_HUMAN antibody
- ExoI antibody
All lanes : Anti-Exonuclease 1 antibody (ab95068) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 30 seconds
ab95068 at 0.4 µg/ml detecting Exonuclease 1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of Exonuclease 1
Lane 2: ab95068 at 10µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
This product has been referenced in:
- Chan YW et al. Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations. Nat Cell Biol 20:92-103 (2018). Read more (PubMed: 29255170) »
- Gong Y et al. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev 31:46-58 (2017). WB ; Mouse . Read more (PubMed: 28115467) »