Anti-EXOSC10/RRP6 antibody (ab94981)
Key features and details
- Rabbit polyclonal to EXOSC10/RRP6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EXOSC10/RRP6 antibody
See all EXOSC10/RRP6 primary antibodies -
Description
Rabbit polyclonal to EXOSC10/RRP6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EXOSC10/RRP6 aa 496-864.
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Positive control
- WB: 293T, H1299 or Raji whole cell lysate. ICC/IF: A549 cells. IHC-P: Cal27 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 101 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 101 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA. -
Sequence similarities
Contains 1 3'-5' exonuclease domain.
Contains 1 HRDC domain. -
Cellular localization
Cytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. - Information by UniProt
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Database links
- Entrez Gene: 5394 Human
- Omim: 605960 Human
- SwissProt: Q01780 Human
- Unigene: 632368 Human
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Alternative names
- Autoantigen PM/Scl 2 antibody
- Exosc10 antibody
- Exosome component 10 antibody
see all
Images
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Anti-EXOSC10/RRP6 antibody (ab94981) at 1/3000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 101 kDa
7.5% SDS PAGE -
1/100 ab94981 staining EXOSC10/RRP6 in a paraffin-embedded Cal27 xenograft.
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1) ab94981 at a 1/200 dilution, staining EXOSC10/RRP6 in paraformaldehyde-fixed A549 cells.
2) image merged with a DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab94981 has been referenced in 2 publications.
- Ma L et al. The m6A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol 38:101801 (2020). PubMed: 33232910
- Haas G et al. Identification of factors involved in target RNA-directed microRNA degradation. Nucleic Acids Res 44:2873-87 (2016). WB ; Human . PubMed: 26809675