Overview

  • Product name

    Anti-EXOSC10/RRP6 antibody
    See all EXOSC10/RRP6 primary antibodies
  • Description

    Rabbit polyclonal to EXOSC10/RRP6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis, Pufferfish, Zebrafish, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to EXOSC10/RRP6 aa 231-245 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    LDVPPALADFIHQQR-C

  • Positive control

    • ICC/IF: Rat NRK, mouse NIH3T3 cells. WB: nuclear exracts of HeLa cells.
  • General notes

     This product was previously labelled as EXOSC10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab50558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 23166521
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). Minor additional bands may be detected in some cell preparations.

Target

  • Function

    Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.
  • Sequence similarities

    Contains 1 3'-5' exonuclease domain.
    Contains 1 HRDC domain.
  • Cellular localization

    Cytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.
  • Information by UniProt
  • Database links

  • Alternative names

    • Autoantigen PM/Scl 2 antibody
    • Exosc10 antibody
    • Exosome component 10 antibody
    • EXOSX_HUMAN antibody
    • P100 polymyositis scleroderma overlap syndrome associated autoantigen antibody
    • P100 polymyositis-scleroderma overlap syndrome-associated autoantigen antibody
    • p2 antibody
    • p3 antibody
    • p4 antibody
    • PM Scl antibody
    • PM/Scl 100 antibody
    • PM/Scl-100 antibody
    • PMSCL antibody
    • PMSCL2 antibody
    • Polymyositis/scleroderma autoantigen 100 kDa antibody
    • Polymyositis/scleroderma autoantigen 2 100 kDa antibody
    • Polymyositis/scleroderma autoantigen 2 antibody
    • RRP6 antibody
    • Rrp6p antibody
    see all

Images

  • Lanes 1-2 : Anti-EXOSC10/RRP6 antibody (ab50558) at 1 µg/ml
    Lane 3 : Anti-EXOSC10/RRP6 antibody (ab50558) at 2 µg/ml

    Lanes 1 & 3 : HeLa nuclear extract
    Lane 2 : HeLa nuclear extract with PM/Scl-100 peptide

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate with a chemiluminescent substrate.

    Predicted band size: 101 kDa
    Observed band size: 101 kDa

  • ab50558 staining EXOSC10/RRP6 in murine NIH3T3 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in 3.7% formaldehyde and permeabilized overnight in 70% ethanol at -20°C and then 30 minutes with 0.5% Triton. Samples were then blocked and incubated with ab50558 at a 1/435 dilution for 30 minutes at 20°C. The secondary used was a goat anti-rabbit polyclonal conjugated to Alex Fluor 594, used at a 1/1000 dilution. Nuclei stained with DAPI.

    See Abreview

References

This product has been referenced in:

  • Davidson L  et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep 26:2779-2791.e5 (2019). Read more (PubMed: 30840897) »
  • Jamin SP  et al. EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis. Sci Rep 7:15065 (2017). WB, IHC-P, IHC (PFA fixed) ; Mouse, Rat . Read more (PubMed: 29118343) »
See all 15 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Zebrafish Tissue lysate - other (Ovary)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
Ovary
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 07 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Permeabilization
Yes - overnight in 70% ethanol at -20, and 30 min in 0.5% Triton-X
Specification
NIH3T3
Blocking step
Roche blocking reagent as blocking agent for 30 minute(s) · Concentration: 1.35% · Temperature: 20°C
Fixative
Formaldehyde

Dr. Jonathan Houseley

Verified customer

Submitted Jul 02 2011

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