Product nameAnti-EXOSC10/RRP6 antibody
See all EXOSC10/RRP6 primary antibodies
DescriptionRabbit polyclonal to EXOSC10/RRP6
Tested applicationsSuitable for: IP, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis, Pufferfish, Zebrafish, Cynomolgus monkey
- ICC/IF: Rat NRK, mouse NIH3T3 cells. WB: nuclear exracts of HeLa cells.
This product was previously labelled as EXOSC10
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 23166521|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). Minor additional bands may be detected in some cell preparations.|
FunctionPutative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.
Sequence similaritiesContains 1 3'-5' exonuclease domain.
Contains 1 HRDC domain.
Cellular localizationCytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.
- Information by UniProt
- Autoantigen PM/Scl 2 antibody
- Exosc10 antibody
- Exosome component 10 antibody
Lanes 1-2 : Anti-EXOSC10/RRP6 antibody (ab50558) at 1 µg/ml
Lane 3 : Anti-EXOSC10/RRP6 antibody (ab50558) at 2 µg/ml
Lanes 1 & 3 : HeLa nuclear extract
Lane 2 : HeLa nuclear extract with PM/Scl-100 peptide
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate with a chemiluminescent substrate.
Predicted band size: 101 kDa
Observed band size: 101 kDa
ab50558 staining EXOSC10/RRP6 in murine NIH3T3 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in 3.7% formaldehyde and permeabilized overnight in 70% ethanol at -20°C and then 30 minutes with 0.5% Triton. Samples were then blocked and incubated with ab50558 at a 1/435 dilution for 30 minutes at 20°C. The secondary used was a goat anti-rabbit polyclonal conjugated to Alex Fluor 594, used at a 1/1000 dilution. Nuclei stained with DAPI.
This product has been referenced in:
- Davidson L et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep 26:2779-2791.e5 (2019). Read more (PubMed: 30840897) »
- Jamin SP et al. EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis. Sci Rep 7:15065 (2017). WB, IHC-P, IHC (PFA fixed) ; Mouse, Rat . Read more (PubMed: 29118343) »