Key features and details
- Rabbit polyclonal to EXOSC10/RRP6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EXOSC10/RRP6 antibody
See all EXOSC10/RRP6 primary antibodies
DescriptionRabbit polyclonal to EXOSC10/RRP6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human EXOSC10/RRP6 aa 496-864.
- WB: 293T, H1299 or Raji whole cell lysate. ICC/IF: A549 cells. IHC-P: Cal27 xenograft.
This product was previously labelled as EXOSC10
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab94981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 101 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
FunctionPutative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.
Sequence similaritiesContains 1 3'-5' exonuclease domain.
Contains 1 HRDC domain.
Cellular localizationCytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.
- Information by UniProt
- Autoantigen PM/Scl 2 antibody
- Exosc10 antibody
- Exosome component 10 antibody
Anti-EXOSC10/RRP6 antibody (ab94981) at 1/3000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 101 kDa
7.5% SDS PAGE
1/100 ab94981 staining EXOSC10/RRP6 in a paraffin-embedded Cal27 xenograft.
1) ab94981 at a 1/200 dilution, staining EXOSC10/RRP6 in paraformaldehyde-fixed A549 cells.
2) image merged with a DNA probe.
ab94981 has been referenced in 1 publication.
- Haas G et al. Identification of factors involved in target RNA-directed microRNA degradation. Nucleic Acids Res 44:2873-87 (2016). WB ; Human . PubMed: 26809675