Overview

  • Product name

    Anti-EXOSC10/RRP6 antibody
    See all EXOSC10/RRP6 primary antibodies
  • Description

    Rabbit polyclonal to EXOSC10/RRP6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human EXOSC10/RRP6 aa 755-804 (internal sequence).
    Sequence:

    ACKAAAEQAISVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKP


    Database link: NP_001001998

  • Positive control

    • Transfected 293T cell lysate
  • General notes

     This product was previously labelled as EXOSC10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab95028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.
  • Sequence similarities

    Contains 1 3'-5' exonuclease domain.
    Contains 1 HRDC domain.
  • Cellular localization

    Cytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.
  • Information by UniProt
  • Database links

  • Alternative names

    • Autoantigen PM/Scl 2 antibody
    • Exosc10 antibody
    • Exosome component 10 antibody
    • EXOSX_HUMAN antibody
    • P100 polymyositis scleroderma overlap syndrome associated autoantigen antibody
    • P100 polymyositis-scleroderma overlap syndrome-associated autoantigen antibody
    • p2 antibody
    • p3 antibody
    • p4 antibody
    • PM Scl antibody
    • PM/Scl 100 antibody
    • PM/Scl-100 antibody
    • PMSCL antibody
    • PMSCL2 antibody
    • Polymyositis/scleroderma autoantigen 100 kDa antibody
    • Polymyositis/scleroderma autoantigen 2 100 kDa antibody
    • Polymyositis/scleroderma autoantigen 2 antibody
    • RRP6 antibody
    • Rrp6p antibody
    see all

Images

  • Anti-EXOSC10/RRP6 antibody (ab95028) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size: 101 kDa



    Gel concentration 6-18%
  • Anti-EXOSC10/RRP6 antibody (ab95028) at 1/1000 dilution + U2OS whole cell lysate at 100 µg

    Predicted band size: 101 kDa

    See Abreview

References

This product has been referenced in:

  • Domanski M & LaCava J Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells. Bio Protoc 7:N/A (2017). Read more (PubMed: 28691041) »
  • Domanski M  et al. Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels. Biotechniques 0:1-6 (2012). Read more (PubMed: 22668517) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Loading amount
100 µg
Specification
U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Jul 20 2012

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