Recombinant
RabMAb

Recombinant Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

Overview

  • Product name

    Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal
    See all EXOSC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14840(B)] to EXOSC3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human EXOSC3 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9NQT5

  • Positive control

    • 293T, HeLa, Jurkat and Raji cell lysates; Human colon tissue; Human transitional cell carcinoma of bladder tissue; 293T and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14840(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/50 - 1/70.
ICC/IF 1/100.

Target

  • Relevance

    EXOSC3 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. It is required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity. [Swiss-Prot]
  • Cellular localization

    Cytoplasm. Nucleus, nucleolus.
  • Database links

  • Alternative names

    • bA3J10.7 antibody
    • CGI 102 antibody
    • CGI-102 antibody
    • Exosome complex component RRP40 antibody
    • Exosome complex exonuclease RRP40 antibody
    • Exosome component 3 antibody
    • hRrp 40 antibody
    • hRrp-40 antibody
    • hRrp40 antibody
    • hRrp40p antibody
    • p10 antibody
    • PCH1B antibody
    • Ribosomal RNA processing protein 40 antibody
    • Ribosomal RNA-processing protein 40, S. cerevisiae, homolog of antibody
    • RP11-3J10.8 antibody
    • RRP 40 antibody
    • Rrp40 antibody
    • Rrp40p antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling EXOSC3 with ab184961 at 1/100 dilution (red). Counterstained with Dapi (blue). 

  • Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling EXOSC3 with ab184961 at 1/100 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • All lanes : Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961) at 1/10000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : Raji cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunoprecipitation analysis of 293T lysates (Lane 1) labeling EXOSC3 with ab184961 at a 1/50 dilution and a negative control PBS (Lane 2).  Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1500 dilution.

  • Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EXOSC3 with ab184961 at 1/50 dilution.  Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human colon tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

ab184961 has not yet been referenced specifically in any publications.

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