Recombinant
RabMAb

Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Overview

  • Product name
    Anti-EXOSC3 antibody [EPR14841] - C-terminal
    See all EXOSC3 primary antibodies
  • Description
    Rabbit monoclonal [EPR14841] to EXOSC3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EXOSC3 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9NQT5

  • Positive control
    • RAW264.7, 293T, Jurkat, HeLa, NIH 3T3, mouse kidney tissue and mouse spleen tissue lysate. Rat kidney tissue lysate. Rat spleen tissue lysate. Human ovarian carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14841
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/30.
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance
    EXOSC3 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. It is required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity. [Swiss-Prot]
  • Cellular localization
    Cytoplasm. Nucleus, nucleolus.
  • Database links
  • Alternative names
    • bA3J10.7 antibody
    • CGI 102 antibody
    • CGI-102 antibody
    • Exosome complex component RRP40 antibody
    • Exosome complex exonuclease RRP40 antibody
    • Exosome component 3 antibody
    • hRrp 40 antibody
    • hRrp-40 antibody
    • hRrp40 antibody
    • hRrp40p antibody
    • p10 antibody
    • PCH1B antibody
    • Ribosomal RNA processing protein 40 antibody
    • Ribosomal RNA-processing protein 40, S. cerevisiae, homolog of antibody
    • RP11-3J10.8 antibody
    • RRP 40 antibody
    • Rrp40 antibody
    • Rrp40p antibody
    see all

Images

  • All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : RAW264.7 cell line lysate
    Lane 6 : NIH 3T3 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde fixed RAW264.7 cells labeling EXOSC3 with ab190689 at 1/250, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and counterstained with DAPI.

  • Lane 1: Primary: ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.

    Lane 2: Primary: ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.



    All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

    Lane 1 : Lysate of 293T cells immunoprecipated with ab190689 at 1/50
    Lane 2 : PBS

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Observed band size: 30 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.

  • Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.

  • All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

    Lane 1 : Jurkat cell line lysate
    Lane 2 : HeLa cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution + 293T cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

References

ab190689 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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