Overview

  • Product name
  • Description
    Rabbit polyclonal to EXOSC5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-185 of Human EXOSC5 (UniProt Q9NQT4).

  • Positive control
    • HeLa cells; Human BT483 xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
  • Tissue specificity
    Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis.
  • Sequence similarities
    Belongs to the RNase PH family.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chronic myelogenous leukemia tumor antigen 28 antibody
    • CML28 antibody
    • EXOS5_HUMAN antibody
    • EXOSC 5 antibody
    • Exosc5 antibody
    • Exosome complex component rrp46 antibody
    • exosome complex exonuclease RRP46 antibody
    • Exosome component 5 antibody
    • exosome component Rrp46 antibody
    • hRrp46p antibody
    • p12B antibody
    • Ribosomal RNA processing protein 46 antibody
    • Ribosomal RNA processing protein 46, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 46 antibody
    • RRP41B antibody
    • RRP46 antibody
    • Rrp46p antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EXOSC5 with ab154718 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled red.
  • Immunohistochemical analysis of paraffin-embedded BT483 xenograft labeling EXOSC5 with ab154718 at 1/500 dilution.

References

ab154718 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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