Overview

  • Product name

    Anti-EXOSC5/CML28 antibody
    See all EXOSC5/CML28 primary antibodies
  • Description

    Rabbit polyclonal to EXOSC5/CML28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human EXOSC5/CML28 aa 1-185.
    Database link: Q9NQT4

  • Positive control

    • HeLa cells; Human BT483 xenograft.
  • General notes

     This product was previously labelled as EXOSC5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
  • Tissue specificity

    Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis.
  • Sequence similarities

    Belongs to the RNase PH family.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chronic myelogenous leukemia tumor antigen 28 antibody
    • CML28 antibody
    • EXOS5_HUMAN antibody
    • EXOSC 5 antibody
    • Exosc5 antibody
    • Exosome complex component rrp46 antibody
    • exosome complex exonuclease RRP46 antibody
    • Exosome component 5 antibody
    • exosome component Rrp46 antibody
    • hRrp46p antibody
    • p12B antibody
    • Ribosomal RNA processing protein 46 antibody
    • Ribosomal RNA processing protein 46, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 46 antibody
    • RRP41B antibody
    • RRP46 antibody
    • Rrp46p antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EXOSC5/CML28 with ab154718 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled red.

  • All lanes : Anti-EXOSC5/CML28 antibody (ab154718) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa nuclear cell lysate

    Lysates/proteins at 30 µg per lane.
  • Immunocytochemistry/ Immunofluorescence of HeLa cells staining EXOSC5/CML28 with ab154718 at 1/500. (green)

    Phalloidin, a cytoskeleton marker, diluted at 1:100. (red)
    Hoechst 33342 staining. (blue)

  • Immunohistochemical analysis of paraffin-embedded BT483 xenograft labeling EXOSC5/CML28 with ab154718 at 1/500 dilution.

References

ab154718 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab154718.
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