Anti-EXOSC5/CML28 antibody (ab168804)
Key features and details
- Rabbit polyclonal to EXOSC5/CML28
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EXOSC5/CML28 antibody
See all EXOSC5/CML28 primary antibodies -
Description
Rabbit polyclonal to EXOSC5/CML28 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human EXOSC5/CML28 aa 1-50. (Uniprot: Q9NQT4)
Database link: NP_064543.3 -
Positive control
- 293T, HeLa and Jurkat cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 25 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. -
Tissue specificity
Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis. -
Sequence similarities
Belongs to the RNase PH family. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56915 Human
- Omim: 606492 Human
- SwissProt: Q9NQT4 Human
- Unigene: 283741 Human
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Alternative names
- Chronic myelogenous leukemia tumor antigen 28 antibody
- CML28 antibody
- EXOS5_HUMAN antibody
see all
Images
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Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. 1) ab168804 (6 µg/mg lysate). 2) A rabbit anti-EXOSC5/CML28 antibody immunoprecipitated EXOSC5/CML28. 3) Control IgG. For blotting ab168804 was used at 1 µg/ml. Detection by chemiluminescence with an exposure time of 10 seconds.
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All lanes : Anti-EXOSC5/CML28 antibody (ab168804) at 0.1 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab168804 has been referenced in 4 publications.
- Chiu H et al. Zinc Finger Protein ZFP36L1 Inhibits Flavivirus Infection by both 5'-3' XRN1 and 3'-5' RNA-Exosome RNA Decay Pathways. J Virol 96:e0166521 (2022). PubMed: 34643435
- Chiu HP et al. Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein. PLoS Pathog 14:e1007166 (2018). PubMed: 30016363
- Lima WF et al. RNA cleavage products generated by antisense oligonucleotides and siRNAs are processed by the RNA surveillance machinery. Nucleic Acids Res 44:3351-63 (2016). WB ; Human . PubMed: 26843429
- Aly HH et al. RNA Exosome Complex Regulates Stability of the Hepatitis B Virus X-mRNA Transcript in a Non-stop-mediated (NSD) RNA Quality Control Mechanism. J Biol Chem 291:15958-74 (2016). WB ; Human . PubMed: 27281821