Overview

  • Product name

    Anti-EXOSC5/CML28 antibody
    See all EXOSC5/CML28 primary antibodies
  • Description

    Rabbit polyclonal to EXOSC5/CML28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human EXOSC5/CML28 aa 1-50. (Uniprot: Q9NQT4)
    Database link: NP_064543.3

  • Positive control

    • 293T, HeLa and Jurkat cell lysates.
  • General notes

     This product was previously labelled as EXOSC5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
  • Tissue specificity

    Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis.
  • Sequence similarities

    Belongs to the RNase PH family.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chronic myelogenous leukemia tumor antigen 28 antibody
    • CML28 antibody
    • EXOS5_HUMAN antibody
    • EXOSC 5 antibody
    • Exosc5 antibody
    • Exosome complex component rrp46 antibody
    • exosome complex exonuclease RRP46 antibody
    • Exosome component 5 antibody
    • exosome component Rrp46 antibody
    • hRrp46p antibody
    • p12B antibody
    • Ribosomal RNA processing protein 46 antibody
    • Ribosomal RNA processing protein 46, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 46 antibody
    • RRP41B antibody
    • RRP46 antibody
    • Rrp46p antibody
    see all

Images

  • Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. 1) ab168804 (6 µg/mg lysate). 2) A rabbit anti-EXOSC5/CML28 antibody immunoprecipitated EXOSC5/CML28. 3) Control IgG. For blotting ab168804 was used at 1 µg/ml. Detection by chemiluminescence with an exposure time of 10 seconds.

  • All lanes : Anti-EXOSC5/CML28 antibody (ab168804) at 0.1 µg/ml

    Lane 1 : 293T cell lysate at 50 µg
    Lane 2 : 293T cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 25 kDa


    Exposure time: 3 minutes

References

This product has been referenced in:

  • Chiu HP  et al. Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein. PLoS Pathog 14:e1007166 (2018). Read more (PubMed: 30016363) »
  • Lima WF  et al. RNA cleavage products generated by antisense oligonucleotides and siRNAs are processed by the RNA surveillance machinery. Nucleic Acids Res 44:3351-63 (2016). WB ; Human . Read more (PubMed: 26843429) »
See all 3 Publications for this product

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