Overview

  • Product name

    Anti-EXOSC5/CML28 antibody
    See all EXOSC5/CML28 primary antibodies
  • Description

    Mouse polyclonal to EXOSC5/CML28
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human EXOSC5/CML28. (AAH07742.1)

  • Positive control

    • human kidney tissue lysate, EXOSC5/CML28 transfected 293T cell lysate.
  • General notes

     This product was previously labelled as EXOSC5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
  • Tissue specificity

    Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis.
  • Sequence similarities

    Belongs to the RNase PH family.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chronic myelogenous leukemia tumor antigen 28 antibody
    • CML28 antibody
    • EXOS5_HUMAN antibody
    • EXOSC 5 antibody
    • Exosc5 antibody
    • Exosome complex component rrp46 antibody
    • exosome complex exonuclease RRP46 antibody
    • Exosome component 5 antibody
    • exosome component Rrp46 antibody
    • hRrp46p antibody
    • p12B antibody
    • Ribosomal RNA processing protein 46 antibody
    • Ribosomal RNA processing protein 46, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 46 antibody
    • RRP41B antibody
    • RRP46 antibody
    • Rrp46p antibody
    see all

Images

  • Anti-EXOSC5/CML28 antibody (ab69699) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 25 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-EXOSC5/CML28 antibody (ab69699) at 1/500 dilution

    Lane 1 : EXOSC5/CML28 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 25 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?

  • ab69699 at 10µg/ml staining EXOSC5/CML28 in human Hela cells by ICC/IF.

References

This product has been referenced in:

  • Cammas A  et al. Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation. Nat Commun 5:4190 (2014). Read more (PubMed: 24969639) »
  • Basu U  et al. The RNA Exosome Targets the AID Cytidine Deaminase to Both Strands of Transcribed Duplex DNA Substrates. Cell 144:353-63 (2011). WB ; Human, Mouse . Read more (PubMed: 21255825) »
See all 2 Publications for this product

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