Overview

  • Product name
    Anti-EXOSC6 antibody
  • Description
    Rabbit polyclonal to EXOSC6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: LYAADEEEAP GTRDPTRLRP VYARAGLLSQ AKGSAYLEAG GTKVLCAVSG, corresponding to amino acids 19-68 of Human EXOSC6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    EXOSC6 constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known; however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • EAP4 antibody
      • Exosome component 6 antibody
      • hMtr3 antibody
      • hMtr3p antibody
      • homolog of yeast mRNA transport regulator 3 antibody
      • Mtr3 (mRNA transport regulator 3)-homolog antibody
      • MTR3 antibody
      • Mtr3p antibody
      • p11 antibody
      see all

    Images

    • Western blot - Anti-EXOSC6 antibody (ab50910)
      Western blot - Anti-EXOSC6 antibody (ab50910)
      Lane 1 : Marker
      Lane 2 : Anti-EXOSC6 antibody (ab50910) at 1 µg/ml

      Lane 2 : Jurkat cell lysate at 10 µg

      Secondary
      Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 28 kDa



      Gel concentration 12%

    References

    ab50910 has not yet been referenced specifically in any publications.

    Publishing research using ab50910? Please let us know so that we can cite the reference in this datasheet.

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