Anti-EXOSC6 antibody (ab50910)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-EXOSC6 antibody
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DescriptionRabbit polyclonal to EXOSC6
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
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Immunogen
A region within synthetic peptide: LYAADEEEAP GTRDPTRLRP VYARAGLLSQ AKGSAYLEAG GTKVLCAVSG, corresponding to amino acids 19-68 of Human EXOSC6
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Positive control
- Jurkat cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab50910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay: 1/62500. |
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| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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RelevanceEXOSC6 constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known; however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 118460 Human
- Entrez Gene: 72544 Mouse
- Entrez Gene: 326786 Zebrafish
- Omim: 606490 Human
- SwissProt: Q5RKV6 Human
- Unigene: 660633 Human
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Alternative names
- EAP4 antibody
- Exosome component 6 antibody
- hMtr3 antibody
see all
Images
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Western blot - Anti-EXOSC6 antibody (ab50910)Lane 1 : Marker
Lane 2 : Anti-EXOSC6 antibody (ab50910) at 1 µg/ml
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Gel concentration 12%
Datasheets and documents
Protocols
References
ab50910 has not yet been referenced specifically in any publications.
