Overview

  • Product name

  • Description

    Rabbit polyclonal to EXOSC8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-261 of Human EXOSC8 (Uniprot: Q96B26).

  • Positive control

    • HepG2, HeLa, HCT116 and A549 whole cell lysates; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.
  • Sequence similarities

    Belongs to the RNase PH family.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA421P11.3 antibody
    • CBP interacting protein 3 antibody
    • CIP3 antibody
    • EAP2 antibody
    • EC 3.1.13 antibody
    • EXOS8_HUMAN antibody
    • EXOSC8 antibody
    • Exosome complex component RRP43 antibody
    • Exosome complex exonuclease RRP43 antibody
    • Exosome component 8 antibody
    • OIP-2 antibody
    • OIP2 antibody
    • Opa interacting protein 2 antibody
    • Opa-interacting protein 2 antibody
    • OTTHUMP00000042274 antibody
    • p9 antibody
    • Ribosomal RNA processing protein 43 antibody
    • Ribosomal RNA processing protein 43, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 43 antibody
    • RP11 421P11.3 antibody
    • RRP43 antibody
    • Rrp43p antibody
    see all

Images

  • Anti-EXOSC8 antibody (ab151584) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling EXOSC8 with ab151584 at 1/500 dilution (green). Alpha-tubulin filaments are labeled red.

References

ab151584 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151584.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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