Key features and details
- Rabbit polyclonal to EXOSC8
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EXOSC8 antibody
See all EXOSC8 primary antibodies
DescriptionRabbit polyclonal to EXOSC8
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- IHC-P: Human Fallopian tube tissue; WB: Transfected U-2 OS whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 30 kDa.|
FunctionNon-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.
Sequence similaritiesBelongs to the RNase PH family.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus.
- Information by UniProt
- bA421P11.3 antibody
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Immunohistochemical analysis of human Fallopian tube tissue labeling EXOSC8 in the cytoplasm and membrane of glandular cells with ab187905 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-EXOSC8 antibody (ab187905) at 0.4 µg/ml
Lane 1 : Transfected target specific siRNA probe #1 into U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 2 : Transfected target specific siRNA probe #2 into U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 3 : Transfected control siRNA into U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Predicted band size: 30 kDa
Remaining relative intensity is presented in image bottom (%).
ab187905 has not yet been referenced specifically in any publications.