Key features and details
- Mouse monoclonal [2337C3a] to EXOSC9
- Suitable for: Dot blot, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-EXOSC9 antibody [2337C3a]
See all EXOSC9 primary antibodies
DescriptionMouse monoclonal [2337C3a] to EXOSC9
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human EXOSC9 (internal sequence).
This product was previously labelled as Exosome Component 9
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityProtein G purified
Purification notesab54283 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab54283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.|
FunctionNon-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs.
Sequence similaritiesBelongs to the RNase PH family.
Cellular localizationNucleus > nucleolus; Cytoplasm. Nucleus > nucleolus and Nucleus. Excluded from the nucleolus.
- Information by UniProt
- Autoantigen PM/Scl 1 antibody
- EXOS9_HUMAN antibody
- EXOSC 9 antibody
Staining of recombinant immunising protein using anti-EXOSC9 antibody [2337C3a] (ab54283).
Predicted band size: 49 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab54283 has not yet been referenced specifically in any publications.