Overview

  • Product name

  • Description

    Goat polyclonal to EXOSC9
  • Host species

    Goat
  • Specificity

    ab85703 is expected to recognize both reported isoforms (NP_001029366.1; NP_005024.2).
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human EXOSC9 aa 412-424 (C terminal).
    Sequence:

    C-RTQTTSAKQEKAP


    Database link: NP_005024.2

  • Positive control

    • Jurkat cell lysate.
  • General notes

     This product was previously labelled as Exosome Component 9

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab85703 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22262661
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 51 kDa). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Alderuccio, J Exp Med. 1991 Apr 1;173(4):941-52; PMID: 2007859).

Target

  • Function

    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs.
  • Sequence similarities

    Belongs to the RNase PH family.
  • Cellular localization

    Nucleus > nucleolus; Cytoplasm. Nucleus > nucleolus and Nucleus. Excluded from the nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Autoantigen PM/Scl 1 antibody
    • EXOS9_HUMAN antibody
    • EXOSC 9 antibody
    • EXOSC9 antibody
    • Exosome complex component RRP45 antibody
    • Exosome complex exonuclease RRP45 antibody
    • Exosome component 9 antibody
    • p5 antibody
    • p6 antibody
    • P75 polymyositis scleroderma overlap syndrome associated autoantigen antibody
    • P75 polymyositis-scleroderma overlap syndrome-associated autoantigen antibody
    • PM/Scl 75 antibody
    • PM/Scl-75 antibody
    • PMSCL autoantigen 75kD antibody
    • PMSCL1 antibody
    • Polymyositis/scleroderma autoantigen 1 75kDa antibody
    • Polymyositis/scleroderma autoantigen 1 antibody
    • Polymyositis/scleroderma autoantigen 75 kDa antibody
    • RRP45 antibody
    • Rrp45p antibody
    see all

Images

  • Anti-EXOSC9 antibody (ab85703) at 0.3 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

    The observed molecular weight corresponds to earlier findings in literature with different antibodies.

References

This product has been referenced in:

  • Pfeiffer JR & Brooks SA Cullin 4B Is Recruited to Tristetraprolin-Containing Messenger Ribonucleoproteins and Regulates TNF-a mRNA Polysome Loading. J Immunol 188:1828-39 (2012). ICC/IF . Read more (PubMed: 22262661) »
See 1 Publication for this product

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