Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (ab228564)

Overview

  • Product name

    Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9)
  • Detection method

    Fluorescent
  • Sample type

    Cell culture media
  • Species reactivity

    Reacts with: Human
  • Product overview

    Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab228564) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.


    Using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody, the kit provides reproducible results and can be run in parallel to exosome immunophenotyping.


    The kit and the measurement of exosomes is useful in the identification of apoptotic cells by flow cytometry.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.

  • Platform

    Flow cytometer

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 25 tests
    Anti-CD9 antibody [VJ1/20] (Biotin) 1 x 125µl
    Assay buffer 10X 1 x 10ml
    Streptavidin-Phycoerythrin 1 x 125µl
    Superparamagnetic capture beads (CD63[TEA3/18]) 1 x 2500µl

Images

  • Flow cytometric analysis of cell culture exosomes, pre-enriched using total exosome isolation from PC-3 cell culture media (A), were resuspended in PBS and bound to CD63-capture beads during an overnight incubation. The following day the bead-bound exosomes were indirect stained with ab228564 and Streptavidin-PE and analyzed by flow cytometry.

  • Dynamic range of the assay analyzed by flow cytometry.

    Relationship between background noise and specific signal at different exosome concentrations.

Protocols

References

ab228564 has not yet been referenced specifically in any publications.

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