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    exosome-isolation-and-analysis-kit-flow-cytometry-cell-culture-cd63--cd9-ab267478.pdf

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Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (ab267478)

  • Datasheet
  • SDS
  • Protocol Booklet
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Key features and details

  • Detection method: Fluorescent
  • Platform: Flow cytometer
  • Sample type: Cell culture media

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Overview

  • Product name

    Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9)
  • Detection method

    Fluorescent
  • Sample type

    Cell culture media
  • Species reactivity

    Reacts with: Human
  • Product overview

    Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab267478) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.


    Using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody, the kit provides reproducible results and can be run in parallel to exosome immunophenotyping.


    The kit and the measurement of exosomes is useful in the identification of apoptotic cells by flow cytometry.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.

  • Platform

    Flow cytometer

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 25 tests
    Anti-CD9 PE (Clone VJ1/20) 1 x 125µl
    Assay Buffer 10X 1 x 10ml
    Superparamagnetic capture beads (CD63[TEA3/18]) 1 x 1250µl

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab267478? Please let us know so that we can cite the reference in this datasheet.

ab267478 has not yet been referenced specifically in any publications.

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