Product nameAnti-Exportin 4/XPO4 antibody [EPR4442(2)]
See all Exportin 4/XPO4 primary antibodies
DescriptionRabbit monoclonal [EPR4442(2)] to Exportin 4/XPO4
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Exportin 4/XPO4 aa 1050-1150 (C terminal).
Database link: Q0VG75
- Jurkat, HeLa, SW480, C6, Raw264.7 and PC12 cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 127 kDa.|
|IP||1/10 - 1/100.|
FunctionMediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Sequence similaritiesBelongs to the exportin family.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- Exp4 antibody
- Exportin-4 antibody
- exportin4 antibody
All lanes : Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : C6 cell lysates
Lane 5 : Raw264.7 cell lysates
Lane 6 : PC12 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 127 kDa
ab133237 has not yet been referenced specifically in any publications.