Recombinant
RabMAb

Recombinant Anti-Exportin-5 antibody [EPR8452] (ab129006)

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Overview

  • Product name

    Anti-Exportin-5 antibody [EPR8452]
    See all Exportin-5 primary antibodies

  • Description

    Rabbit monoclonal [EPR8452] to Exportin-5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Exportin-5 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, C6, RAW264.7, PC12, and NIH 3T3 cell lysates; Human fetal heart and brain lysates; Human colon tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab129006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi).
      Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.

    • Tissue specificity

      Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.

    • Sequence similarities

      Belongs to the exportin family.

    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Exp 5 antibody
        • Exp5 antibody
        • Exportin 5 antibody
        • Exportin-5 antibody
        • Exportin5 antibody
        • FLJ14239 antibody
        • FLJ32057 antibody
        • FLJ45606 antibody
        • KIAA1291 antibody
        • OTTHUMP00000016472 antibody
        • Ran binding protein 21 antibody
        • Ran-binding protein 21 antibody
        • Ranbp 21 antibody
        • Ranbp21 antibody
        • Xpo 5 antibody
        • xpo5 antibody
        • XPO5_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Exportin-5 antibody [EPR8452] (ab129006)
        Western blot - Anti-Exportin-5 antibody [EPR8452] (ab129006)
        All lanes : Anti-Exportin-5 antibody [EPR8452] (ab129006) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Human fetal heart lysate
        Lane 4 : Human fetal brain lysate
        Lane 5 : C6 cell lysate
        Lane 6 : RAW264.7 cell lysate
        Lane 7 : PC12 cell lysate
        Lane 8 : NIH 3T3 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 136 kDa
        Observed band size: 136 kDa

      • Flow Cytometry - Anti-Exportin-5 antibody [EPR8452] (ab129006)
        Flow Cytometry - Anti-Exportin-5 antibody [EPR8452] (ab129006)
        Overlay histogram showing HEK293 cells stained with ab129006 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129006, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Exportin-5 antibody [EPR8452] (ab129006)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Exportin-5 antibody [EPR8452] (ab129006)

        ab129006, at 1/250 dilution, staining Exportin-5 in paraffin-embedded Human colon tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Other - Anti-Exportin-5 antibody [EPR8452] (ab129006)
        Other - Anti-Exportin-5 antibody [EPR8452] (ab129006)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      Publishing research using ab129006? Please let us know so that we can cite the reference in this datasheet.

      ab129006 has been referenced in 1 publication.

      • Nuovo GJ  et al. Importin-ß and exportin-5 are indicators of acute viral infection: Correlation of their detection with commercially available detection kits. Ann Diagn Pathol 34:36-41 (2018). PubMed: 29661725

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      I received and tested the new vial. I still have the same problem as with the previous one...

      best regards,

      Read More
      Answer

      Thank you for your email. I am sorry the results hasn't been improved.

      We have received no other compliant for this antibody so I can confirm that we are not aware of any quality problem with this antibody. It may be that the Santa cruz antibody is giving no specific results however it will be difficult to judge.

      Could you kindly send me the protocol you are using? May be an update in protocol could help.

      I will look forward to hearing from you soon.

      Read More

      Question

      The antibody working perfectly in WB.
      It seems it is binding in correctly to cytoplasm.

      Read More
      Answer

      Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

      I look forward to receiving your reply.

      Read More

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