Product nameAnti-Exportin-5 antibody [EPR8453]
See all Exportin-5 primary antibodies
DescriptionRabbit monoclonal [EPR8453] to Exportin-5
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Exportin-5 aa 1150-1250. The exact sequence is proprietary.
- HeLa, K562 and 293T cell lysates. A431 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.39 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 136 kDa.|
FunctionMediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi).
Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.
Tissue specificityExpressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the exportin family.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- Exp 5 antibody
- Exp5 antibody
- Exportin 5 antibody
All lanes : Anti-Exportin-5 antibody [EPR8453] (ab131281) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 136 kDa
Immunofluorescence analysis of A431 cells labelling Exportin-5 with ab131281 at 1/100 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Ghatak S et al. Barrier Function of the Repaired Skin Is Disrupted Following Arrest of Dicer in Keratinocytes. Mol Ther 23:1201-1210 (2015). Read more (PubMed: 25896246) »
- Xie M et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). Read more (PubMed: 24360278) »