Overview

  • Product name

    Anti-Exportin-5 antibody [EPR8453]
    See all Exportin-5 primary antibodies
  • Description

    Rabbit monoclonal [EPR8453] to Exportin-5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Exportin-5 aa 1150-1250. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562 and 293T cell lysates. A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi).
      Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.
    • Tissue specificity

      Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the exportin family.
    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Exp 5 antibody
      • Exp5 antibody
      • Exportin 5 antibody
      • Exportin-5 antibody
      • Exportin5 antibody
      • FLJ14239 antibody
      • FLJ32057 antibody
      • FLJ45606 antibody
      • KIAA1291 antibody
      • OTTHUMP00000016472 antibody
      • Ran binding protein 21 antibody
      • Ran-binding protein 21 antibody
      • Ranbp 21 antibody
      • Ranbp21 antibody
      • Xpo 5 antibody
      • xpo5 antibody
      • XPO5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Exportin-5 antibody [EPR8453] (ab131281) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 136 kDa

    • Immunofluorescence analysis of A431 cells labelling Exportin-5 with ab131281 at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mingyi Xie

    Verified customer

    Submitted Jul 05 2013

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