Key features and details
- Rabbit polyclonal to Extracellular matrix protein 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Extracellular matrix protein 1 antibody
See all Extracellular matrix protein 1 primary antibodies
DescriptionRabbit polyclonal to Extracellular matrix protein 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Extracellular matrix protein 1 aa 386-540.
HPPSPTRDECFARRAPYPNYDRDILTIDIGRVTPNLMGHLCGNQRVLTKH KHIPGLIHNMTARCCDLPFPEQACCAEEEKLTFINDLCGPRRNIWRDPAL CCYLSPGDEQVNCFNINYLRNVALVSGDTENAKGQGEQGSTGGTNISSTS EPKEE
Database link: Q16610
- WB: MCF7 whole cell lysate. IHC-P: Human tonsil tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab234976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
|WB||1/500 - 1/5000.|
FunctionInvolved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
Tissue specificityExpressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.
Involvement in diseaseDefects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- ECM 1 antibody
- Ecm1 antibody
- ECM1_HUMAN antibody
Paraffin-embedded human tonsil tissue stained for Extracellular matrix protein 1 using ab234976 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Anti-Extracellular matrix protein 1 antibody (ab234976) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234976 has not yet been referenced specifically in any publications.