Recombinant
RabMAb

Recombinant Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

Overview

  • Product name

    Anti-Extracellular matrix protein 1 antibody [EPR6701]
    See all Extracellular matrix protein 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1
  • Host species

    Rabbit
  • Specificity

    The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Extracellular matrix protein 1 aa 100-200. The exact sequence is proprietary.
    Database link: Q16610

  • Positive control

    • WB: MDA MB 435 cell line, Rat heart, Rat kidney, Human skin cell, and A375 cell line lysates. IHC-P: Human breast carcinoma and Human kidney tissues. ICC/IF: A375 cells. IP: A375 cells. FC: A375 cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

See IHC antigen retrieval protocols.

Flow Cyt 1/20.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
  • Tissue specificity

    Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.
  • Involvement in disease

    Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • ECM 1 antibody
    • Ecm1 antibody
    • ECM1_HUMAN antibody
    • Extracellular matrix protein 1 antibody
    • Secretory component p85 antibody
    • URBWD antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.01 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/2000 dilution (Purified)

    Lane 1 : A375 (Human malignant melanoma epithelial cell)
    Lane 2 : Rat heart lysates
    Lane 3 : Rat kidney lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • ab126629 (purified) at 1:20 dilution (2µg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
    Lane 1 (input): A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab126629 & A375 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

  • All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/1000 dilution

    Lane 1 : MDA MB 435 cell line lysate
    Lane 2 : Human skin cell lysate
    Lane 3 : A375 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibodty at 1/2000 dilution

    Predicted band size: 61 kDa

  • ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Li W  et al. Exosomal FMR1-AS1 facilitates maintaining cancer stem-like cell dynamic equilibrium via TLR7/NF?B/c-Myc signaling in female esophageal carcinoma. Mol Cancer 18:22 (2019). Read more (PubMed: 30736860) »
  • Ziegler YS  et al. Integration of Breast Cancer Secretomes with Clinical Data Elucidates Potential Serum Markers for Disease Detection, Diagnosis, and Prognosis. PLoS One 11:e0158296 (2016). WB . Read more (PubMed: 27355404) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton-x
Specification
spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 01 2017

Answer

I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

The difference between these two isoforms is that ECM1b lacks amino acids 237-361. Therefore, if you had an antibody targeting this region, it would detect ECM1a but not ECM1b. You however wouldn't be able to specifically detect ECM1b as all the amino acids present in this isoform are also present in ECM1a.

Read More

Answer

Thank you for contacting us.

In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), some against protein fragments and others against the full length protein (either purified or overexpressed in another organism) or a cell preparation.

Which specific antibody or protein target do you have in mind?

As for the anti-IgA antibodies - the immunogendepends on the antibody.

Please let me know more details about the products of your interest and I'd be happy to assist you further.

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