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Do you have specific antibodies recognizing ECM1a and ECM1b independently working in WB or ELISA ?
Asked on Sep 02 2014
I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)
Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.
The difference between these two isoforms is that ECM1b lacks amino acids 237-361. Therefore, if you had an antibody targeting this region, it would detect ECM1a but not ECM1b. You however wouldn't be able to specifically detect ECM1b as all the amino acids present in this isoform are also present in ECM1a.
Elisa ThomasAbcam Scientific Support
Answered on Sep 02 2014