Recombinant Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free
See all Extracellular matrix protein 1 primary antibodies -
Description
Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast carcinoma and Human kidney tissues. ICC/IF: A375 cells. IP: A375 cells. Flow Cyt (intra): A375 cells.
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General notes
ab242391 is the carrier-free version of ab126629.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6701 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
- PE Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab306226)
- APC Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab306227)
- HRP Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab306228)
- Alexa Fluor® 488 Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab309717)
- Alexa Fluor® 647 Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab310083)
- Alexa Fluor® 594 Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab310485)
- Alexa Fluor® 555 Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab312014)
- Alexa Fluor® 568 Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab312491)
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Conjugation kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. -
Tissue specificity
Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. -
Involvement in disease
Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1893 Human
- Entrez Gene: 116662 Rat
- Omim: 602201 Human
- SwissProt: Q16610 Human
- SwissProt: Q62894 Rat
- Unigene: 81071 Human
- Unigene: 97792 Rat
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Alternative names
- ECM 1 antibody
- Ecm1 antibody
- ECM1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.01 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab126629 (purified) at 1:20 dilution (2µg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
Lane 1 (input): A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab126629 & A375 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126629).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126629).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126629).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab242391 has not yet been referenced specifically in any publications.