Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free (ab212329)

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Overview

  • Product name

    Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free
    See all Extracellular matrix protein 1 primary antibodies

  • Description

    Mouse monoclonal [SC05] to Extracellular matrix protein 1 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    ab212329 reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component.

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Full length protein corresponding to Human Extracellular matrix protein 1. Immunogen isolated from Human colostrum.
    Database link: Q16610

  • Positive control

    • Stomach, lung, or breast tumor; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab212329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.

in 0.1 ml
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

for 30 minutes at room temperature.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

  • Tissue specificity

    Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.

  • Involvement in disease

    Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • ECM 1 antibody
      • Ecm1 antibody
      • ECM1_HUMAN antibody
      • Extracellular matrix protein 1 antibody
      • Secretory component p85 antibody
      • URBWD antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free (ab212329)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free (ab212329)

      Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling IgA Secretory Component with ab212329 at 1 ug/ml.

    References

    ab212329 has not yet been referenced specifically in any publications.

    Publishing research using ab212329? Please let us know so that we can cite the reference in this datasheet.

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