Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free (ab212329)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free (ab212329)

    Key features and details

    • Mouse monoclonal [SC05] to Extracellular matrix protein 1 - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Rat, Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free
      See all Extracellular matrix protein 1 primary antibodies

    • Description

      Mouse monoclonal [SC05] to Extracellular matrix protein 1 - BSA and Azide free

    • Host species

      Mouse

    • Specificity

      ab212329 reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component.

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Rat, Human

    • Immunogen

      Full length protein corresponding to Human Extracellular matrix protein 1. Immunogen isolated from Human colostrum.
      Database link: Q16610

    • Positive control

      • Stomach, lung, or breast tumor; Human colon carcinoma tissue.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab212329 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    for 30 minutes at room temperature.

    Target

    • Function

      Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

    • Tissue specificity

      Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.

    • Involvement in disease

      Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • ECM 1 antibody
        • Ecm1 antibody
        • ECM1_HUMAN antibody
        • Extracellular matrix protein 1 antibody
        • Secretory component p85 antibody
        • URBWD antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free (ab212329)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [SC05] - BSA and Azide free (ab212329)

        Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling IgA Secretory Component with ab212329 at 1 ug/ml.

      References (0)

      Publishing research using ab212329? Please let us know so that we can cite the reference in this datasheet.

      ab212329 has not yet been referenced specifically in any publications.

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