Overview

  • Product name

    Extraction Buffer 5X PTR
  • Product overview

    Abcam’s Extraction Buffer 5X is formulated for the preparation of cell or tissues extracts for use in immunoassays.


    The Extraction Buffer should be diluted to 1X in the appropriate buffer before use. If required the Extraction Buffer 5X can be used in conjunction of Abcam’s Extraction Enhancer Buffer 50X (ab193971).

  • Notes

    Instructions for use

    Before use the Extraction Buffer 5X should be diluted to 1X with distilled water. 

    Note: The provided Extraction Buffer 5X contains phosphatase inhibitors and protease inhibitor aprotinin. Additional protease inhibitors can be added if required.

    If the Extraction Buffer 5X is to be used in conjunction with Extraction Enhancer Buffer 50X (ab193971), then follow the instructions below:

    1X Extraction Buffer + Enhancer

    Prepare 1X Extraction Buffer + Enhancer by diluting Extraction Buffer 5X and Extraction Enhancer Buffer 50X  to 1X with deionized water. To make 10 mL 1X Extraction Buffer + Enhancer combine 7.8 mL deionized water, 2 mL Extraction Buffer 5X and 200 µL Extraction Enhancer Buffer 50X.  Mix thoroughly and gently.

Properties

Associated products

References

This product has been referenced in:

  • Fragniere AMC  et al. Hyperosmotic stress induces cell-dependent aggregation of a-synuclein. Sci Rep 9:2288 (2019). Read more (PubMed: 30783136) »
  • Hosseinkhani B  et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). Read more (PubMed: 30131806) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer



I can confirm that the extraction buffer is Triton X-100 based. It does not interfere with BCA protein method at 5X or 1X concentrations. Commercial Bradford assay specifications list Triton as interfering with the assay at 0.05%. Therefore 1X extraction buffer should be diluted at least 5-fold to be compatible with the Bradford method.

I suggest to add an extraction buffer only blank as a control measurement in your experiment.

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