Product nameExtraction Buffer 5X PTR
Abcam’s Extraction Buffer 5X is formulated for the preparation of cell or tissues extracts for use in immunoassays.
The Extraction Buffer should be diluted to 1X in the appropriate buffer before use. If required the Extraction Buffer 5X can be used in conjunction of Abcam’s Extraction Enhancer Buffer 50X (ab193971).
Instructions for use
Before use the Extraction Buffer 5X should be diluted to 1X with distilled water.
Note: The provided Extraction Buffer 5X contains phosphatase inhibitors and protease inhibitor aprotinin. Additional protease inhibitors can be added if required.
If the Extraction Buffer 5X is to be used in conjunction with Extraction Enhancer Buffer 50X (ab193971), then follow the instructions below:
1X Extraction Buffer + Enhancer
Prepare 1X Extraction Buffer + Enhancer by diluting Extraction Buffer 5X and Extraction Enhancer Buffer 50X to 1X with deionized water. To make 10 mL 1X Extraction Buffer + Enhancer combine 7.8 mL deionized water, 2 mL Extraction Buffer 5X and 200 µL Extraction Enhancer Buffer 50X. Mix thoroughly and gently.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 ml 50 ml Extraction Buffer 5X 1 x 10ml 1 x 50ml
This product has been referenced in:
- Fragniere AMC et al. Hyperosmotic stress induces cell-dependent aggregation of a-synuclein. Sci Rep 9:2288 (2019). Read more (PubMed: 30783136) »
- Hosseinkhani B et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). Read more (PubMed: 30131806) »