Anti-EYA1 antibody - N-terminal (ab170649)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-EYA1 antibody - N-terminal
See all EYA1 primary antibodies -
DescriptionRabbit polyclonal to EYA1 - N-terminal
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with: Mouse
Predicted to work with: Human -
Immunogen
Synthetic peptide within Human EYA1 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q99502 -
Positive control
- Mouse bladder tissue lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading...
-
PurityImmunogen affinity purified
-
Purification notesab170649 is purified through a protein A column, followed by peptide affinity purification.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/100 - 1/500. Predicted molecular weight: 65 kDa. |
Target
-
FunctionTyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Seems to coactivate SIX2, SIX4 and SIX5. May be required for normal development of branchial arches, ear and kidney.
-
Tissue specificityIn the embryo, highly expressed in kidney with lower levels in brain. Weakly expressed in lung. In the adult, highly expressed in heart and skeletal muscle. Weakly expressed in brain and liver. No expression in eye or kidney.
-
Involvement in diseaseDefects in EYA1 are the cause of branchiootorenal syndrome type 1 (BOR1) [MIM:113650]; also known as Melnick-Fraser syndrome. BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
Defects in EYA1 are the cause of otofaciocervical syndrome (OFCS) [MIM:166780]. The syndrome is characterized by trophic alterations of the facies and shoulder girdle in addition to the malformations seen in BOR.
Defects in EYA1 are the cause of branchiootic syndrome type 1 (BOS1) [MIM:602588]; also known as BO syndrome type 1 or branchiootic dysplasia. Individuals with BOS1 are affected by the same branchial and otic anomalies as those seen in individuals with BOR1, but lack renal anomalies. -
Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. EYA family.
-
Developmental stageDetected in cytoplasm of somite cells at the beginning of fourth week of development. Detected in cytoplasm of limb bud cell between the sixth and eighth week of development.
-
Post-translational
modificationsSumoylated by SUMO1. -
Cellular localizationCytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
- Information by UniProt
-
Database links
- Entrez Gene: 2138 Human
- Entrez Gene: 14048 Mouse
- Omim: 601653 Human
- SwissProt: Q99502 Human
- SwissProt: P97767 Mouse
- Unigene: 491997 Human
- Unigene: 250185 Mouse
-
Alternative names
- BOP antibody
- BOR antibody
- BOS1 antibody
see all
Images
Protocols
Datasheets and documents
References
ab170649 has not yet been referenced specifically in any publications.