Overview

  • Product name

  • Description

    Rabbit polyclonal to EYA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 100 and 150 of human EYA2 (NP_005235.3)

  • Positive control

    • 293T cells lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab95875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity.
  • Tissue specificity

    Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.
  • Sequence similarities

    Belongs to the HAD-like hydrolase superfamily. EYA family.
  • Developmental stage

    At the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EAB 1 antibody
    • EAB1 antibody
    • EYA 2 antibody
    • EYA transcriptional coactivator and phosphatase 2 antibody
    • EYA2 antibody
    • EYA2_HUMAN antibody
    • eyes absent 2 homolog (Drosophila) antibody
    • Eyes absent homolog 2 (Drosophila) antibody
    • Eyes absent homolog 2 antibody
    • Translation of this uORF probably lowers the translation efficiency of EYA2 antibody
    see all

Images

  • All lanes : Anti-EYA2 antibody (ab95875) at 0.2 µg/ml

    Lane 1 : 293T cell lysate at 50 µg
    Lane 2 : 293T cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA2 with ab95875 at 1/1000 (1µg/ml). Detection: DAB.
  • Detection of human Eya2 by Western Blot of Immunoprecipitate. ab95875 at 0.1µg/ml staining Eya2 in 293T cell lysate immunoprecipitated using ab95875 at 3µg/mg (1mg/IP; 20% of IP loaded/lane) Detection: Chemiluminescence with exposure time of 10 seconds..

References

This product has been referenced in:

  • Xu H  et al. EYA2 Correlates With Clinico-Pathological Features of Breast Cancer, Promotes Tumor Proliferation, and Predicts Poor Survival. Front Oncol 9:26 (2019). Read more (PubMed: 30761270) »
  • Li Z  et al. EYA2 promotes lung cancer cell proliferation by downregulating the expression of PTEN. Oncotarget 8:110837-110848 (2017). Read more (PubMed: 29340020) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. To our knowledge, ab54713 and ab95875 have not been tested in mouse. The immunogen for ab54713 shows a homology of 89% to mouse whereas the immunogen for ab95875 shows a slightly lower homology of 80%. We generally expect cross-reactivity from 85% homology. Therefore, I can offer a discount off a future purchase if you buy ab54713 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab54713 in mouse. I will then send a discount code. This code must be issued before purchasing ab54713 so please wait for my reply before ordering. 2. Purchase ab54713 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab54713 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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