Overview

  • Product name

    Anti-EYA2 antibody [EPR2942]
    See all EYA2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2942] to EYA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal region of Human EYA2 (UniProt ID: O00167).

  • Positive control

    • Y-79 cell lysate and 293T cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/10 - 1/100.
    WB: 1/50000 - 1/200000. Predicted molecular weight: 59 kDa.

    Is unsuitable for Flow Cyt, ICC or IHC.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity.
    • Tissue specificity

      Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.
    • Sequence similarities

      Belongs to the HAD-like hydrolase superfamily. EYA family.
    • Developmental stage

      At the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • EAB 1 antibody
      • EAB1 antibody
      • EYA 2 antibody
      • EYA transcriptional coactivator and phosphatase 2 antibody
      • EYA2 antibody
      • EYA2_HUMAN antibody
      • eyes absent 2 homolog (Drosophila) antibody
      • Eyes absent homolog 2 (Drosophila) antibody
      • Eyes absent homolog 2 antibody
      • Translation of this uORF probably lowers the translation efficiency of EYA2 antibody
      see all

    Images

    • All lanes : Anti-EYA2 antibody [EPR2942] (ab92505) at 1/50000 dilution

      Lane 1 : Y-79 cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 59 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

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