• Product name

  • Description

    Rabbit polyclonal to EYA4
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from within residues: MEDSQDLNEQ SVKKTCTESD VSQSQNSRSM EMQDLASPHT LVGGGDTPGS, corresponding to N terminal amino acids 1-50 of Human EYA4 (NP_004091.3)

  • Positive control

    • HeLa wholec cell lysate



Our Abpromise guarantee covers the use of ab93865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye.
    • Tissue specificity

      Highly expressed in heart and skeletal muscle.
    • Involvement in disease

      Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10) [MIM:601316]. DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
      Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J) [MIM:605362]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Belongs to the HAD-like hydrolase superfamily. EYA family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CMD1J antibody
      • Deafness, autosomal dominant 10 antibody
      • DFNA 10 antibody
      • DFNA10 antibody
      • dJ78N10.1 (eyes absent (Drosophila) homolog 4) antibody
      • dJ78N10.1 (eyes absent) antibody
      • EYA 4 antibody
      • eya4 antibody
      • EYA4_HUMAN antibody
      • Eyes absent 4 antibody
      • Eyes absent homolog 4 (Drosophila) antibody
      • Eyes absent homolog 4 antibody
      • HGNC:3522 antibody
      • OTTHUMP00000040267 antibody
      see all


    • All lanes : Anti-EYA4 antibody (ab93865) at 0.4 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 70 kDa

      Exposure time: 3 minutes
    • 3µg ab93865 were used to immunoprecipitate EYA4 from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with 1µg/ml ab93865.
      Detection: chemiluminescence with exposure time of 10 seconds.


    ab93865 has not yet been referenced specifically in any publications.

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