Product nameAnti-EZH1 antibody
See all EZH1 primary antibodies
DescriptionRabbit polyclonal to EZH1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
- MCF7 cell extracts, Mouse lung, brain, kidney and spleen tissue extracts
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 85 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
Cellular localizationNucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
- Information by UniProt
- Enhancer of zeste homolog 1 (Drosophila) antibody
- Enhancer of zeste homolog 1 antibody
- ENX-2 antibody
Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab189833. Blue DAPI for nuclear staining.
All lanes : Anti-EZH1 antibody (ab189833) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : Mouse lung tissue extract
Lane 3 : Mouse brain tissue extract
Lane 4 : Mouse kidney tissue extract
Lane 5 : Mouse spleen tissue extract
Predicted band size: 85 kDa
ab189833 has not yet been referenced specifically in any publications.