Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EZH1 peptide (ab22954).|
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
Cellular localizationNucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
- Information by UniProt
- Enhancer of zeste homolog 1 (Drosophila) antibody
- Enhancer of zeste homolog 1 antibody
- ENX-2 antibody
Anti-EZH1 antibody (ab3670) at 2 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 85 kDa
Observed band size: 85 kDa
ab3670 staining (2
µg/ml) of MOLT-4 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
This product has been referenced in:
- Luo L et al. Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats. Exp Ther Med 13:645-650 (2017). Read more (PubMed: 28352345) »