• Product name

  • Description

    Rabbit polyclonal to EZH1
  • Host species

  • Specificity

    This antibody detects endogenous levels of total EZH1 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) from an internal region of EZH1.

  • Positive control

    • WB: Extracts from HT-29 cells, LOVO cells, A549 cells, NIH-3T3 cells, RAW264.7 cells and COS-7 cells. IHC-P: human brain tissue.



Our Abpromise guarantee covers the use of ab64850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.


  • Function

    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
  • Information by UniProt
  • Database links

  • Alternative names

    • Enhancer of zeste homolog 1 (Drosophila) antibody
    • Enhancer of zeste homolog 1 antibody
    • ENX-2 antibody
    • ENX2 antibody
    • Ezh1 antibody
    • EZH1_HUMAN antibody
    • Histone-lysine N-methyltransferase EZH1 antibody
    see all


  • All lanes : Anti-EZH1 antibody (ab64850) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extract from LOVO
    Lane 3 : Extracts from A549 cells
    Lane 4 : Extracts from NIH-3T3 cells
    Lane 5 : Extracts from RAW264.7 cells
    Lane 6 : Extracts from COS-7 cells
    Lane 7 : Extracts from COS-7 cells with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa

  • Ab64850 (1/50 dilution) staining EZH1 in paraffin embedded human brain tissue by immunohistochemistry.

    The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.


This product has been referenced in:

  • Wang L  et al. The Effect of Overexpression of the Enhancer of Zeste Homolog 1 (EZH1) Gene on Aristolochic Acid-Induced Injury in HK-2 Human Kidney Proximal Tubule Cells In Vitro. Med Sci Monit 25:801-810 (2019). Read more (PubMed: 30688289) »
  • Koppens MA  et al. Large variety in a panel of human colon cancer organoids in response to EZH2 inhibition. Oncotarget 7:69816-69828 (2016). Read more (PubMed: 27634879) »
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for contacting us. We have three antibodies that may be suitable for your purposes:

ab13665: rabbit polyclonal to EZH1 validated in WB on mouse and human samples
ab86128: rabbit polyclonal to EZH1 validated in WB and ICC/IF on mouse and human samples
ab64850: rabbit polyclonal to EZH1 validated in WB, IHC-P, and ELISA on human and mouse samples

Please let me know if you need any additional information or assistance.

Read More


Thank you for sending the image of your blots.

When you use the PVDF membrane, are you pre-wetting it with methanol? Because PVDF is hydrophobic, it may not properly bind the blocking agent without the pre-wetting step. This could potentially cause the black blot you are seeing.

The speckled signal observed with the donkey anti-rabbit HRP secondary and 5% donkey serum blocking agent is typically a product of contamination in the blocking buffer or primary antibody diluent. This may be prevented by either filtering your reagents or using fresh buffers. This could also bea result of contamination on the sponge in your transfer apparatus. Thoroughly washing the transfer equipment should prevent this.

This EZH1 antibody is validated using 5% BSA in TBST as a blocking agent, but it should be suitable for use with milk and donkey serum as well. Because the signal observed is so strong, I would recommend using a much higher dilution of secondary antibody (at least 1:50k - 1:100k). Increasing the blocking time to overnight at 4C may also help. If you are looking to try an alternative blocking agent, gelatin is sometimes used. There are also several commercial blocking agents available (including ab126587 and ab64234), however these tend to be casein based. Because casein is the major protein in milk, they may or may not produce different results in your experiment.

I hope this helps, please let me know if you need any additional information or assistance.

Read More


Thank you for your phone call. As we discussed, it is possible that your 6 x 15 minute washes are washing the blocking agent off your membrane. It may help to reduce the wash time to 3 x 5 min, shorten the primary antibody incubation to 1 hour at RT, and use less primary antibody. I hope this will help to improve your results, if not, please let me know and I will be happy to help you further.

Read More


Thank you for contacting us. We do not have tests against purified Ezh1 or Ezh2 but a BLAST search of the immunogen sequence returns only Ezh1, so it is expected that the antibody is not capable of detecting Ezh2. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

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