Anti-EZH1 antibody - N-terminal (ab176115)
Key features and details
- Rabbit polyclonal to EZH1 - N-terminal
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-EZH1 antibody - N-terminal
See all EZH1 primary antibodies -
Description
Rabbit polyclonal to EZH1 - N-terminal -
Host species
Rabbit -
Specificity
Multiple isoforms of EZH1 are known to exist. EZH1 ab176115 is predicted to not cross-react with EZH2
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Human EZH1 aa 180-230 (N terminal). The exact sequence is proprietary.
Database link: Q92800 -
Positive control
- WB: Mouse lung tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab176115 is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa. |
Target
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Function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
Cellular localization
Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3. - Information by UniProt
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Database links
- Entrez Gene: 14055 Mouse
- SwissProt: P70351 Mouse
- Unigene: 5027 Mouse
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Alternative names
- Enhancer of zeste homolog 1 (Drosophila) antibody
- Enhancer of zeste homolog 1 antibody
- ENX-2 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab176115 has been referenced in 4 publications.
- Meng TG et al. Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis. Int J Biol Sci 18:4513-4531 (2022). PubMed: 35864958
- Meng TG et al. PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome. Nat Commun 11:6354 (2020). PubMed: 33311485
- Agarwal P et al. Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells. Cell Stem Cell 24:769-784.e6 (2019). PubMed: 30905620
- Li J et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). PubMed: 24162653